Recombinant Anti-BCAR1 (phospho Y410) antibody [EPR1860] (2024)

Overview

• Product name

  Anti-BCAR1 (phospho Y410) antibody [EPR1860] - BSA and Azide free
  See all BCAR1 primary antibodies

• Description

  Rabbit monoclonal [EPR1860] to BCAR1 (phospho Y410) - BSA and Azide free

• Host species

  Rabbit

• Tested applications

  Suitable for: WBmore details
  Unsuitable for: Flow Cyt,ICC/IF or IHC-P

• Species reactivity

  Reacts with: Human
  

• Immunogen

  Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

• General notes

  ab247593 is the carrier-free version of ab92699.

  Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

  See Also

  Anti-BCAR1 (phospho Y751) antibody (ab45486)Anti-BCAR1 Antibodies | InvitrogenAnti-BCAR1 antibody (ab137507) | AbcamAnti-BCAR1 antibody [M144] (ab31831) | Abcam

  This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

  Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

  This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

  This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free production

  For more information see here.

  Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

  Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

• Form

  Liquid

• Storage instructions

  Shipped at 4°C. Store at +4°C. Do Not Freeze.

• Storage buffer

  pH: 7.2
  Constituent: PBS

• Carrier free

  Yes

• Concentration information loading...

• Purity

  Affinity purified

• Clonality

  Monoclonal

• Clone number

  EPR1860

• Isotype

  IgG

• Research areas

  • Signal Transduction
  • Protein Phosphorylation
  • Tyrosine Kinases
  • Other

  • Cancer
  • Signal transduction
  • Other

Associated products

• Alternative Versions

  • Anti-BCAR1 (phospho Y410) antibody [EPR1860] (ab92699)

• Conjugation kits

  • HRP Conjugation Kit - Lightning-Link® (ab102890)

• Isotype control

  • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)

• Recombinant Protein

  • Recombinant Human BCAR1 protein (ab132142)

Applications

Target

• Function

  Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.

• Tissue specificity

  Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line.

• Sequence similarities

  Belongs to the CAS family.
  Contains 1 SH3 domain.

• Domain

  Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL1 SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with NEDD9.
  A serine-rich region promotes activation of the serum response element (SRE).
  The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of PTK2/FAK11.

• Post-translational
  modifications

  PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix.
  Dephosphorylated by PTPN14 at Tyr-128.

• Cellular localization

  Cell junction, focal adhesion. Cytoplasm. Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation.

• Information by UniProt

• Database links

  • Entrez Gene: 9564 Human
  • Omim: 602941 Human
  • SwissProt: P56945 Human
  • Unigene: 479747 Human

• Alternative names

  • BCAR 1 antibody
  • Bcar1 antibody
  • BCAR1_HUMAN antibody

  see all

Images

Protocols

Datasheets and documents

References (0)