Anti-BCAR1 (phospho Y751) antibody (ab45486) (2024)

Overview

• Product name

  Anti-BCAR1 (phospho Y751) antibody
  See all BCAR1 primary antibodies

• Description

  Rabbit polyclonal to BCAR1 (phospho Y751)

• Host species

  Rabbit

• Specificity

  The antibody detects a 130 kDa protein corresponding to the molecular mass of phosphorylated p130 Cas on SDS-PAGE immunoblots of pervanadate treated human endothelial and A431 cells, but is not observed in control cells.

• Tested applications

  Suitable for: WBmore details

  See Also

  Anti-BCAR1 Antibodies | InvitrogenAnti-BCAR1 antibody (ab137507) | AbcamRecombinant Anti-BCAR1 (phospho Y410) antibody [EPR1860]Anti-BCAR1 antibody [M144] (ab31831) | Abcam

• Species reactivity

  Reacts with: Human
  Predicted to work with: Mouse

• Immunogen

  Synthetic peptide corresponding to Rat BCAR1 (phospho Y751) conjugated to keyhole limpet haemocyanin.
  Database link: Q63767

• Positive control

  • Pervanadate treated human endothelial and A431 cells

• General notes

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

• Form

  Liquid

• Storage instructions

  Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

• Storage buffer

  Preservative: 0.05% Sodium azide
  Constituents: PBS, 50% Glycerol, 0.1% BSA

• Concentration information loading...

• Purity

  Immunogen affinity purified

• Purification notes

  This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose and to phospho-p130 Cas (Tyr-762) peptide before affinity purification using phospho-p130 Cas (Tyr-751) peptide (without carrier).

• Clonality

  Polyclonal

• Isotype

  IgG

• Research areas

  • Signal Transduction
  • Protein Phosphorylation
  • Tyrosine Kinases
  • Other

  • Cancer
  • Signal transduction
  • Other

Associated products

• Compatible Secondaries

• Isotype control

  • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

• Recombinant Protein

  • Recombinant Human BCAR1 protein (ab132142)

Applications

Target

• Function

  Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.

• Tissue specificity

  Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line.

• Sequence similarities

  Belongs to the CAS family.
  Contains 1 SH3 domain.

• Domain

  Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL1 SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with NEDD9.
  A serine-rich region promotes activation of the serum response element (SRE).
  The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of PTK2/FAK11.

• Post-translational
  modifications

  PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix.
  Dephosphorylated by PTPN14 at Tyr-128.

• Cellular localization

  Cell junction, focal adhesion. Cytoplasm. Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation.

• Information by UniProt

• Database links

  • Entrez Gene: 9564 Human
  • Entrez Gene: 12927 Mouse
  • Omim: 602941 Human
  • SwissProt: P56945 Human
  • SwissProt: Q61140 Mouse
  • Unigene: 479747 Human
  • Unigene: 3758 Mouse

• Alternative names

  • BCAR 1 antibody
  • Bcar1 antibody
  • BCAR1_HUMAN antibody

  see all

Images

Protocols

Datasheets and documents

References (0)