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Overview
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Product name
Anti-BCAR1 antibody
See all BCAR1 primary antibodies -
Description
Rabbit polyclonal to BCAR1
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Host species
Rabbit
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Tested applications
Suitable for: WB, ICC/IFmore details
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Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow -
Immunogen
Recombinant fragment, corresponding to a region within internal sequence amino acids 250-710 of Human BCAR1 (UniProt ID: P56945).
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Positive control
- A431, H1299, HeLa, HepG2 and NIH 3T3 cell lysates; methanol fixed A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid
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Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified
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Clonality
Polyclonal
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Isotype
IgG
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Research areas
- Signal Transduction
- Protein Phosphorylation
- Tyrosine Kinases
- Other
- Cancer
- Signal transduction
- Other
Associated products
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Compatible Secondaries
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Isotype control
- Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
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Positive Controls
- HeLa whole cell lysate (ab29545)
- NIH/3T3 whole cell lysate (ab7179)
- A-431 whole cell lysate (ab7909)
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Recombinant Protein
- Recombinant Human BCAR1 protein (ab132142)
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Related Products
- Recombinant Human BCAR1 protein (ab132142)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137507 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/500 - 1/3000. Predicted molecular weight: 93 kDa. | |
ICC/IF | 1/100 - 1/1000. |
Notes |
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WB |
ICC/IF |
Target
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Function
Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.
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Tissue specificity
Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line.
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Sequence similarities
Belongs to the CAS family.
Contains 1 SH3 domain. -
Domain
Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL1 SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with NEDD9.
A serine-rich region promotes activation of the serum response element (SRE).
The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of PTK2/FAK11. -
Post-translational
modificationsPTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix.
Dephosphorylated by PTPN14 at Tyr-128. -
Cellular localization
Cell junction, focal adhesion. Cytoplasm. Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation.
- Information by UniProt
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Database links
- Entrez Gene: 527550 Cow
- Entrez Gene: 9564 Human
- Entrez Gene: 12927 Mouse
- Entrez Gene: 25414 Rat
- Omim: 602941 Human
- SwissProt: P56945 Human
- SwissProt: Q61140 Mouse
- SwissProt: Q63767 Rat
see all
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Alternative names
- BCAR 1 antibody
- Bcar1 antibody
- BCAR1_HUMAN antibody
see all
Images
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Western blot - Anti-BCAR1 antibody (ab137507)
Anti-BCAR1 antibody (ab137507) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 93 kDa
7.5% SDS PAGE
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Western blot - Anti-BCAR1 antibody (ab137507)
Anti-BCAR1 antibody (ab137507) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg
Predicted band size: 93 kDa
7.5% SDS PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-BCAR1 antibody (ab137507)
Confocal immunofluorescence analysis of methanol fixed A431 cells labelling BCAR1 with ab137507 (green) at 1/500 dilution. Alpha tubulin filaments were labeled with an Alpha tubulin antibody (red) at 1/500 dilution.
Protocols
Click here to view the general protocols
Datasheets and documents
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SDS download
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Datasheet download
Download
References (0)
Publishing research using ab137507? Please let us know so that we can cite the reference in this datasheet.
ab137507 has not yet been referenced specifically in any publications.